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UTX knockdown affects the MLL complex activity at RAR target genes. Downregulation of UTX impaired trimethyl H3K4 (3meH3K4) levels (A) and ASH2L occupancy (B) at RAR target genes. shRandom or shUTX U937 cells that were untreated (−RA) or treated with RA (+RA) were subjected to ChIP analysis, using the indicated antibodies. IgG was included as a control. Error bars indicate standard deviations obtained from three independent experiments.
