FIG 2.

Effects of lentivirus-mediated KD of energy-sensing pathway components on myogenesis. (A) Effects of KD on expression of the total levels of the indicated targets in normal muscle infected with lentivirus expressing the indicated shRNA for 3 days. (B to G) Injured rat quadriceps muscle was infected with lentivirus expressing shRNA targeting the specified mRNA or nontargeted (NT) shRNA on day 3 and then treated with pcRNA1-3 on day 6. (B) Hematoxylin and eosin (H&E) stain of injury site 2 weeks after treatment with pcRNA; scale bar, 100 μm. (C) Isolectin B4 staining of endothelial cells (green) at injury site at 2 weeks, merged with the differential interference contrast (DIC) image (gray) and nuclear DAPI stain (blue); scale bar, 10 μm. (D to G) Quantification (means ± standard deviations [SD]) of the number of myofibers (D), myofiber cross-sectional area (E), number of nuclei (F), and isometric contraction force (ICF, in milliNewtons) (G) of muscle infected with lentivirus targeting the indicated mRNA after treatment with pcRNAs for 2 weeks.