FIG 4.

Effects of KD of energy-sensing pathway components on the satellite cell differentiation program. Injury site muscle infected with lentivirus targeting the indicated mRNA or expressing nontargeted (NT) shRNA (top of each panel), at the indicated number of days following pcRNA treatment, was analyzed by Western blotting using antibodies specific to the proteins indicated on the right side. (A) Levels of the indicated proteins in KD muscle at various times, compared to the NT controls. Time zero represents injured muscle prior to pcRNA treatment; n, normal muscle. (B) Time course of expression of PCNA and MRFs in control or KD muscle at various times. (C) Levels of total or phospho-4EBP1 or-S6K1 in control and KD muscle at indicated times following pcRNA treatment.