FIG 1.

Gcn5 and PCAF function redundantly to regulate adipogenesis. (A to D) Gcn5 and PCAF function redundantly for H3K9ac and adipogenesis in brown preadipocytes. Brown preadipocytes were isolated from Gcn5^flox/flox and Gcn5^flox/flox; PCAF^−/− mice and immortalized with SV40T prior to Gcn5 deletion by retroviral Cre. Subconfluent cells were subjected to qRT-PCR analysis of Gcn5 and PCAF mRNA (A) and immunoblotting with the antibodies indicated on the right (B). The cells were induced to undergo adipogenesis for 6 days, followed by Oil Red O staining (C) and qRT-PCR analysis of gene expression (D). (E) Gcn5 and PCAF are required for development of BAT in vivo. Gcn5^flox/flox; PCAF^−/− males were crossed with Gcn5^flox/+; PCAF^−/−; Myf5-Cre females to generate Gcn5^flox/flox; PCAF^−/−; Myf5-Cre pups. Sagittal sections of E16.5 embryos were subjected to H&E staining (upper panels) and immunofluorescence staining with antibodies against myosin and UCP1 (lower panels). Myosin (red) and UCP1 (green) are specifically expressed in skeletal muscle and BAT, respectively.