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. 2014 Oct;34(19):3702–3720. doi: 10.1128/MCB.00099-14

FIG 6.

FIG 6

H3K9 and H3K4 methylation status of target genes are regulated by hypoxia, Jmjd1a, and G9a. Representative data from 3 independent rounds of ChIP assays performed with anti-H3K4me3 or anti-H3K9me2 antibodies quantified in Igfbp4 (A), Notch4 (B), and Robo4 (C) gene promoter loci by Q-PCR and expressed as fold change values over the average of control IgG at 1 for all panels (n = 3 technical replicates). (D) The Mlh1 promoter region was used as a negative control for the ChIP assays, as it displays no significant change in H3K4 trimethylation status in the presence (WT Normoxia) or absence (Jmjd1a KO Normoxia) of Jmjd1a. Horizontal bars, Q-PCR amplicons on promoter regions; boxes, exons with UTRs in white and coding regions in black; vertical bars, TATA boxes and putative hypoxia-responsive elements (HRE).