FIG 2.

FACS analysis of E17.5 Sec23b^gt/gt and WT FLC. (A) Livers were harvested from 3 Sec23b^gt/gt and 3 WT E17.5 embryos. Flow cytometry on FLC single-cell suspensions demonstrated equivalent total numbers of recovered cells from Sec23b^gt/gt and WT fetal livers. Each liver is represented by 1 point, and horizontal lines indicate mean values for each group. (B) The number of long-term hematopoietic stem cells (ckit⁺ Sca1⁺ CD48⁻ CD150⁺ Lin⁻) was equivalent for Sec23b^gt/gt and WT fetal livers. (C) FACS distributions used to calculate numbers of long-term hematopoietic stem cells in panel B.