FIG 6.

Analysis of peripheral blood and BM hematopoietic compartments of mice cotransplanted with a 1:1 mix of GFP⁻ Sec23b^gt/gt FLC and UBC-GFP^Tg+ Sec23b^+/+ FLC (experimental arm) and control mice cotransplanted with a 1:1 mix of GFP⁻ and GFP^Tg+ Sec23b^+/+ FLC. (A to C) By peripheral blood FACS, Mac1⁺ Gr1⁺ neutrophils (A), B220⁺ B lymphocytes (B), and CD3⁺ T lymphocytes (C) were found to be derived from both Sec23b^gt/gt and WT FLC. The Sec23b^gt/gt peripheral blood cells persisted at a stable level throughout the 18-week follow-up period, suggesting no competitive advantage to WT FLC over Sec23b^gt/gt FLC at reconstituting hematopoiesis. (D and E) In the BM, the contributions of GFP⁻ Sec23b^gt/gt FLC to the long-term hematopoietic stem cells (D; ckit⁺ Sca1⁺ CD48⁻ CD150⁻ Lin⁻) and to myeloid cells (E; Mac1⁺ GR1⁺) in the experimental arm were equivalent to the contributions of the GFP⁻ WT cells in the control arm. (F) Similarly, the contribution of GFP(-) Sec23b^gt/gt T lymphocytes in the thymus was equivalent to that of GFP⁻ WT cells in the control arm. *, there was a trend for some subsets of GFP⁻ Sec23b^gt/gt T lymphocytes to be underrepresented; however, this did not reach statistical significance after correction for multiple observations, based on the Holm-Sidak method or the Bonferroni method. ISP, immature single-positive cells; DP, CD4⁺ CD8⁺ double-positive T lymphocytes; NS, not significant. (G) In contrast, GFP⁻ Sec23b^gt/gt CD19⁺ CD220⁺ BM B lymphocytes were underrepresented. Each point represents one mouse. Lines represent mean values for each group, and error bars indicate standard deviations.