FIG 7.
Reconstituted GFP− hematopoietic cells in mice cotransplanted with a 1:1 mix of GFP− Sec23bgt/gt FLC and UBC-GFPTg+ Sec23b+/+ FLC were derived from Sec23bgt/gt FLC and not from host reconstitution. GFP− myeloid cells isolated from mice transplanted with a 1:1 ratio of UBC-GFPtg/+ FLC and Sec23bgt/gt FLC and from control mice transplanted with 1:1 ratio of UBC-GFPtg/+ FLC and WT FLC were genotyped for Sec23bgt/gt. Six mice from each group were examined. Each lane represents the genotype of myeloid cells isolated from a single mouse. The genotype of a Sec23b+/gt control DNA is shown. The lower and upper bands correspond to the expected PCR products for the Sec23b WT and gt alleles, respectively.