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. 2014 Oct;34(19):3721–3734. doi: 10.1128/MCB.00287-14

TABLE 1.

Genotyping and qPCR primer sequences

Primer Primer sequence (5′ → 3′)
Sec23b F1 ATAGACCAGGCTGGCCTCAGTC
Sec23b cgt B1 CCACAACGGGTTCTTCTGTT
Sec23b cgt R1 CGAGCACAGAGAGACCCAAT
Sec23b R CAAGTGAGTGCCTCCTCACA
Sec23b F2 AACAGCCCAGGTGACTAGGA
Sec23b qPCR F CCCTACGTCTTTCAGATTGTCA
Sec23b qPCR R CGGGCAAAATGGTGTCTATAA
Sec24a qPCR F GAGCAGAGATGGAGCGTTCCT
Sec24a qPCR R TTCTTCCAACCCAAAGCATCA
Sec24b qPCR F GACCCGAGAAGGCGCTTT
Sec24b qPCR R TTTGCCAACCCAAATGTAGAAA
Sec24c qPCR F TTATGCGGGTTCGGACAAG
Sec24c qPCR R CTCATATAGAAAGCGCCAAAGAAAT
Sec24d qPCR F CTCATATAGAAAGCGCCAAAGAAAT
Sec24d qPCR R TCATGTACACAGGCAGCACCTT
Sar1a qPCR F TCGGTGGGCATGAGCAA
Sar1a qPCR R GCCATTAATCGCTGGGAGATAA
Sar1b qPCR F GGGTGGGCACGTGCAA
Sar1b qPCR R TGCCATTGATAGCAGGAAGGT
TRAPPC3 qPCR F GCACGGAGAGCAAGAAAATGA
TRAPPC3 qPCR R GGTGACAAGCGCTCCATAGG
Beta-actin qPCR F GATCTGGCACCACACCTTCT
Beta-actin qPCR R GGGGTGTTGAAGGTCTCAAA
GAPDH qPCR F TGTGTCCGTCGTGGATCTGA
GAPDH qPCR R ACCACCTTCTTGATGTCATCATACTT