TABLE 2.
Microbial detection in spiked islet samples and corresponding saline controls by the BacT/Alert system using PF (aerobic) and BPN (anaerobic) bottlesa
| Organism | Sample type | BacT/Alert PF culture bottle |
BacT/Alert BPN culture bottle |
||
|---|---|---|---|---|---|
| Detection time (h) (mean ± SD) | P value | Detection time (h) (mean ± SD) | P value | ||
| S. epidermidis | Transport fluid | 29.3 ± 7.4 | 0.3210 | 17.4 ± 2.5 | 0.2839 |
| Saline (transport fluid control) | 34.2 ± 1.3 | 19.2 ± 0.6 | |||
| Postpurification | 32.6 ± 0.9 | 0.6218 | 19.9 ± 1.4 | 0.5956 | |
| Saline (postpurification control) | 34.3 ± 3.9 | 19.4 ± 0.7 | |||
| Pretransplant | 26.9 ± 0.4 | 0.0528 | Negative | — | |
| Saline (pretransplant control) | 34.7 ± 3.5 | 19.1 ± 1.0 | |||
| C. albicans | Transport fluid | 21.0 ± 7.1 | 0.2584 | 16.1 ± 2.1 | 0.0023 |
| Saline (transport fluid control) | 54.9 ± 43.9 | 26.3 ± 1.5 | |||
| Postpurification | 27.8 ± 0.5 | 0.3466 | 26.6 ± 2.7 | 0.8149 | |
| Saline (postpurification control) | 43.2 ± 24.7 | 26.2 ± 1.8 | |||
| Pretransplant | 29.1 ± 0.9 | 0.2421 | 25.3 ± 0.2 | 0.8249 | |
| Saline (pretransplant control) | 31.5 ± 2.9 | 25.5 ± 2.0 | |||
| E. coli | Transport fluid | 12.9 ± 0.3 | 0.3635 | 11.7 ± 0.3 | 1.0000 |
| Saline (transport fluid control) | 13.4 ± 0.7 | 11.7 ± 0.5 | |||
| Postpurification | 13.7 ± 0.7 | 0.4068 | 11.7 ± 0.5 | 0.5979 | |
| Saline (postpurification control) | 14.2 ± 0.5 | 11.9 ± 0.2 | |||
| Pretransplant | 15.3 ± 0.6 | 0.0761 | Negative | — | |
| Saline (pretransplant control) | 14.2 ± 0.4 | 12.1 ± 0.1 | |||
a
n = 3 for all samples; P values are from an unpaired t test.