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. 2014 Oct;52(10):3614–3623. doi: 10.1128/JCM.01653-14

TABLE 2.

RT-PCR and quantitative real-time RT-PCR results for samples collected from 13 dead green anaconda juveniles that underwent necropsy

Anaconda juvenile no. Sample collection date (day/mo/yr) Sample identificationa AnaPV RT-PCR result Viral load (RNA copies per mg tissue or ml fecal sample)
001 19/10/2011 1110RN041/stomach mucus −
19/10/2011 1110RN041/lung/liver/kidney/spleen + 1.34 × 106
002 19/10/2011 1110RN043/liver −
19/10/2011 1110RN043/kidney + 4.48 × 104
19/10/2011 1110RN043/spleen −
003 31/10/2011 1110RN047/kidney −
31/10/2011 1110RN047/intestine + 1.74 × 107
31/10/2011 1110RN047/brain −
31/10/2011 1110RN047/gallbladder −
31/10/2011 1110RN047/stomach −
31/10/2011 1110RN047/esophagus −
31/10/2011 1110RN047/liver −
31/10/2011 1110RN047/lungs −
31/10/2011 1110RN047/heart −
31/10/2011 1110RN047/tracheas −
31/10/2011 1110RN047/brain −
31/10/2011 1110RN047/lung/liver/kidney/spleen −
013 8/1/2012 1201RN001/heart −
8/1/2012 1201RN001/lung + 2.30 × 106
8/1/2012 1201RN001/liver + 3.62 × 105
8/1/2012 1201RN001/kidney −
8/1/2012 1201RN001/brain −
8/1/2012 1201RN001/stomach + 2.33 × 104
8/1/2012 1201RN001/intestine + 7.85 × 107
8/1/2012 1201RN001/skin + 1.07 × 107
035 27/12/2011 1112RN058/heart −
27/12/2011 1112RN058/lung + 1.26 × 105
27/12/2011 1112RN058/liver + 1.29 × 106
27/12/2011 1112RN058/spleen −
27/12/2011 1112RN058/pancreas + 3.03 × 105
27/12/2011 1112RN058/kidney + 2.34 × 107
264 21/1/2012 1201RN003/spleen −
21/1/2012 1201RN003/heart + 1.06 × 106
21/1/2012 1201RN003/brain −
21/1/2012 1201RN003/small intestine and feces + 1.05 × 108
21/1/2012 1201RN003/kidney + 4.23 × 107
21/1/2012 1201RN003/lung + 5.57 × 106
21/1/2012 1201RN003/liver + 1.88 × 107
21/1/2012 1201RN003/granuloma on stomach + 7.40 × 105
535 7/12/2011 1112RN052/skin + 5.52 × 106
7/12/2011 1112RN052/kidney + 5.65 × 106
7/12/2011 1112RN052/liver + 9.92 × 104
7/12/2011 1112RN052/thyroid + 3.85 × 107
7/12/2011 1112RN052/heart −
7/12/2011 1112RN052/lung + 5.53 × 107
7/12/2011 1112RN052/spleen −
7/12/2011 1112RN052/gastric + 1.03 × 107
7/12/2011 1112RN052/fecal swab −
7/12/2011 1112RN052/intestine + 3.07 × 107
7/12/2011 1112RN052/brain −
554 15/12/2011 1112RN053/heart −
15/12/2011 1112RN053/kidney + 2.37 × 107
15/12/2011 1112RN053/intestine + 3.37 × 107
15/12/2011 1112RN053/liver + 8.23 × 105
15/12/2011 1112RN053/lung + 1.24 × 107
15/12/2011 1112RN053/skin −
15/12/2011 1112RN053/subcutaneous lesion −
15/12/2011 1112RN053/stomach −
15/12/2011 1112RN053/trachea −
573 5/5/2012 1205RN015/colon + 4.70 × 106
5/5/2012 1205RN015/pancreas + 2.19 × 106
5/5/2012 1205RN015/kidney + 2.57 × 106
5/5/2012 1205RN015/skin −
5/5/2012 1205RN015/lungs + 7.84 × 106
5/5/2012 1205RN015/heart −
5/5/2012 1205RN015/liver −
5/5/2012 1205RN015/spleen −
5/5/2012 1205RN015/duodenum + 4.42 × 107
620 22/11/2011 1111RN049/fecal swab + 1.90 × 108
22/11/2011 1111RN049/intestine + 4.19 × 105
22/11/2011 1111RN049/kidney + 3.54 × 107
22/11/2011 1111RN049/liver −
22/11/2011 1111RN049/lung −
771 10/1/2012 1201RN002/GI tract + 2.45 × 105
10/1/2012 1201RN002/liver + 7.40 × 104
10/1/2012 1201RN002/lung + 2.13 × 106
10/1/2012 1201RN002/heart −
10/1/2012 1201RN002/kidney + 3.92 × 106
10/1/2012 1201RN002/spleen + 1.04 × 106
10/1/2012 1201RN002/trachea −
834 13/7/2012 1207RN022/brain −
13/7/2012 1207RN022/heart −
13/7/2012 1207RN022/lung + 1.25 × 107
13/7/2012 1207RN022/stomach −
13/7/2012 1207RN022/spleen −
13/7/2012 1207RN022/liver −
13/7/2012 1207RN022/pancreas −
13/7/2012 1207RN022/left kidney −
13/7/2012 1207RN022/right kidney + 8.46 × 106
849 16/3/2012 1203RN009/brain −
16/3/2012 1203RN009/fecal sample −
16/3/2012 1203RN009/kidney −
16/3/2012 1203RN009/liver −
16/3/2012 1203RN009/lung −
16/3/2012 1203RN009/pericardial fluid −
16/3/2012 1203RN009/spleen −
16/3/2012 1203RN009/stomach −
16/3/2012 1203RN009/subcutaneous necrotic tissue + 1.23 × 106
a

GI, gastrointestinal.