TABLE 2.

Genetic polymorphisms of the seven virulence-associated genes analyzed by MVLST

Gene	Size of PCR product (bp)	Positions of the fragment analyzed within each genea	No. of alleles	No. (%) of polymorphic sites	dN/dSb	dN	dS	Tajima's D testc	SLAC (95% CI)d
atlLR2	650	1879–2418	8	8 (1.47)	0.416	0.0042	0.01	−0.13385	0.647 (0.277–1.250)
atlLR3	590	2419–2859	9	9 (2.04)	0.258	0.0046	0.0179	−0.43781	0.338 (0.121–0.728)
hlb	554	274–714	10	12 (2.72)	0.024	0.0012	0.0471	0.38138	0.092 (0.015–0.285)
isdJ	498	971–1382	14	10 (2.42)	0.103	0.0034	0.033	0.90961	0.166 (0.066–0.336)
SLUG_09050	406	124–457	5	4 (1.20)	0	0	0.0282	0.95707	0 (0–0.203)
SLUG_16930	559	586–1037	14	9 (1.99)	0.256	0.0044	0.0171	0.42415	0.345 (0.158–0.643)
vwbl	461	932–1275	9	7 (2.03)	0.133	0.0025	0.0192	0.70819	0.178 (0.044–0.463)

^aFragment positions are based on the genome of S. lugdunensis N920143.

^bd_N/d_S, ratio of nonsynonymous to synonymous substitutions.

^cP > 0.1 for all Tajima's D test results.

^dSLAC, single-likelihood ancestor counting; CI, confidence interval.