TABLE 2.
Isolates of Rickettsia rickettsii used in the phylogenetic analysis of the present study
| Isolate | Isolation source | Clinical outcome | Source of DNA sequences for rickettsial genes (GenBank accession no. or reference no.) for: |
Haplotypea | Tickb | ||||
|---|---|---|---|---|---|---|---|---|---|
| gltA | RR0155-rpmB | RR1240-tlc5b | cspA-ksgA | ompB | |||||
| Iowa | Tick | CP000766 | CP000766 | CP000766 | CP000766 | CP000766 | A | Dermacentor variabilis | |
| Hino | Human | Fatal | CP003309 | CP003309 | CP003309 | CP003309 | CP003309 | A | D. variabilis |
| Hauke | Human | Fatal | CP003318 | CP003318 | CP003318 | CP003318 | CP003318 | A | D. variabilis |
| Arizona | Human | Fatal | CP003307 | CP003307 | CP003307 | CP003307 | CP003307 | B | Rhipicephalus sanguineus sensu lato |
| Sheila Smith | Human | Fatal | CP000848 | CP000848 | CP000848 | CP000848 | CP000848 | C | Dermacentor andersoni |
| Bitterroot | Tick | RRU59729 | EF216032 | EF215983 | EF215860 | X16353 | C | D. andersoni | |
| Costa Rica | Human | Fatal | 19, 27 | EF216038 | EF215987 | EF215872 | 27 | D | ? |
| I12B (Villeta) | Tick | KJ735644 | KJ735647 | KJ735648 | KJ735649 | KJ735646 | D | Amblyomma patinoi | |
| Rs1 | Tick | 13 | This study | This study | This study | 13 | D | Amblyomma sculptum | |
| Itu | Tick | KF742602 | This study | This study | This study | KF742604 | D | A. sculptum | |
| Taiaçu | Tick | DQ115890 | This study | This study | This study | 12 | D | Amblyomma aureolatum | |
| IAL 1–2c | Human | Fatal | This study | This study | This study | This study | This study | D | A. aureolatum |
| IAL 4, 9d | Human | Cure | This study | This study | This study | This study | This study | D | A. sculptum |
| IAL 3, 5–8, 10–15e | Human | Fatal | This study | This study | This study | This study | This study | D | A. sculptum |
| Brazil | Human | Unknown | CP003305 | CP003305 | CP003305 | CP003305 | CP003305 | D | A. sculptum |
| Colombia | Human | Fatal | CP003306 | CP003306 | CP003306 | CP003306 | CP003306 | E | A. patinoi |
Refers to the concatenated haplotypes shown in the phylogenetic tree (Fig. 1).
For tick isolates, refers to the tick species from which the isolate was obtained; for human isolates, refers to the incriminated vector of R. rickettsii to humans in the area of origin of the isolate, according to Ogrzewalska et al. (32) for Amblyomma aureolatum; A. A. Faccini-Martínez, F. B. Costa, T. E. Hayama-Ueno, A. Ramírez-Hernández, J. Cortés-Vecino, M. B. Labruna, and M. Hidalgo (submitted for publication), Nava et al. (33), and Pacheco et al. (13) for Amblyomma patinoi and Amblyomma sculptum; and Karpathy et al. (10) for Dermacentor variabilis, Dermacentor andersoni, and Rhipicephalus sanguineus sensu lato. The question mark represents an unknown vector, according to Hun et al. (27).
Geographic origins (municipalities in the state of São Paulo, Brazil) of these isolates: São Paulo, IAL 1; São Bernardo do Campo, IAL 2.
Geographic origins (municipalities in the state of São Paulo) of these isolates: Piracicaba, IAL 4; Valinhos, IAL 9.
Geographic origins (municipalities in the state of São Paulo) of these isolates: Valinhos, IAL 3, 5, 8, 11, 12, and 15; Campinas, IAL 6 and 13; Jaguariúna, IAL 7; Piracicaba, IAL 10; Limeira, IAL 14.