TABLE 1.

Strain-independent differential regulation of immune response genes in lungs at 6 h postchallenge^a

Gene symbol	Strain 1 vs resting		Strain 1861 vs resting
	Fold change	Significance	Fold change	Significance
Casp1	2.55	*	4.42	**
Ccr5	3.44	*	3.18	*
Cd14	36.69	*	28.55	***
Cd80	13.00	*	5.97	****
Cd86	2.51	*	4.49	**
Csf2	14.25	***	9.42	****
Icam1	4.37	**	4.05	**
Il1a	12.51	***	20.08	****
Il1b	56.20	**	63.47	****
Il1r	3.62	**	3.31	***
Il23a	36.57	**	51.20	**
Il6	146.02	***	161.79	*
Itgam	6.55	**	12.58	**
Jak2	2.86	*	3.85	*
Nlrp3	38.19	**	54.21	**
Nod2	9.66	*	19.07	*
Slc11a1	3.47	*	2.62	*
Tlr4	2.70	*	5.32	*
Tlr8	2.57	**	2.40	*
Tnf	71.26	*	55.16	*
Tlr5	−2.26	**	−3.69	***

^aAnimals (n = 4 per group) were challenged with 10⁷ CFU of strain 1 or strain 1861 or left unchallenged (“resting”). Lungs were harvested for RNA isolation at 6 h postchallenge, and immune response gene expression was analyzed using mouse innate and adaptive immune response RT² Profiler PCR arrays (cutoff, fold change ≥ 2). Data represent changes in gene expression in lungs from challenged animals relative to resting controls. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.