TABLE 5.

Strain 1861 induces stronger upregulation of genes involved in the IFN-I response than strain 1 compared to resting conditions^a

Gene symbol	Strain 1 vs restingb		Strain 1861 vs restingb		Strain 1861 vs strain 1c
	Fold change	Significance	Fold change	Significance	Fold change	Significance
Ccl2	102.86	****	421.53	**	4.1	**
Ccl4	205.36	**	817.17	****	3.98	***
Cxcl10	1,490.93	*	5,770.58	**	3.87	*
Ddx58	3.1	***	6.21	***	2	**
Eif2ak2	3.94	***	16.62	****	4.22	***
Gbp1	8.95	****	29.33	****	3.28	***
Ifi204	7.78	*	18.82	***	2.42	*
Ifih1	4.69	***	20.39	**	4.35	*
Ifit1	24.37	***	77.82	**	3.19	*
Ifit2	21.25	***	105.02	***	4.94	***
Ifit3	3.09	***	9.22	**	2.98	**
Ifitm1	4.21	***	8.56	**	2.03	*
Il10	7.23	***	14.85	***	2.05	*
Il15	2.7	**	9.95	****	3.69	***
Irf1	5.33	****	11.59	***	2.17	**
Irf7	7.32	**	19.83	***	2.71	**
Isg15	26.3	**	110.24	**	4.19	**
Isg20	3.21	****	7.31	***	2.27	**
Mx1	24.08	*	67.74	****	2.81	*
Mx2	14.12	***	34.05	**	2.41	*
Oas1a	5.59	***	18.76	****	3.36	***
Psme2	2.26	*	5.88	**	2.6	*
Stat1	3.1	*	10.02	***	3.23	**
Stat2	3.38	*	8.98	****	2.66	**

^aAnimals (n = 4 per group) were challenged with 10⁷ CFU of strain 1 or strain 1861 or left unchallenged (“resting”). Lungs were harvested for RNA isolation at 6 h postchallenge, and immune response gene expression was analyzed using mouse type I interferon response RT² Profiler PCR arrays (cutoff used, fold change ≥ 2). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

^bData represent changes in gene expression in lungs from challenged animals relative to resting controls.

^cData represent changes in gene expression in lungs from animals challenged with strain 1861 relative to lungs from animals challenged with strain 1.