FIG 3.

The growth inhibition effect of three C. burnetii effectors was altered in the presence of caffeine. (A) The C. burnetii effectors (indicated on the right) were cloned under the GAL1 promoter and grown on plates containing glucose (Glu), galactose (Gal, inducing conditions), or galactose supplemented with 2 mM caffeine (Gal + Caff) in the wild-type S. cerevisiae BY4741. pGREG523 (Vector) was used as a negative control. (B) The protein levels of the C. burnetii effectors, indicated below each pair, on a galactose-containing plate (left) and a galactose- and caffeine-containing plate (right) were determined by Western analysis using anti-myc antibody. Actin was used as a loading control.