FIG 3.
Toxin-induced changes in secreted chemokines and cytokines in HCT-8 cells. HCT-8 cells were treated with 1 μg/ml (A) or the indicated doses (B) of SubAB, SubAA272B, or Stx2 for 24 h or were left untreated (control). Levels of secreted chemokines or cytokines (as indicated) were assayed using a cytometric bead array (see Materials and Methods). The results are presented as percent changes in RFI relative to levels in control cells. The data are means ± SEM from at least 7 samples from 3 independent experiments. ***, P < 0.001; **, P < 0.01; *, P < 0.05 relative to control cells (Student's unpaired, two-tailed t test).