FIG 1.

miR-21 and miR-181b expression is increased during sepsis. Sepsis was induced by CLP. Whole bone marrow cells were pooled (n = 5 per group) from septic mice that were moribund and were sacrificed at day 3 (representing early sepsis) and day 12 (representing late sepsis). A group of naive mice (day 0) served as a control. (A) miRNA-enriched RNA was isolated from whole bone marrow cells and analyzed by real-time PCR using miR-21- and miR-181b-specific assay primer sets. Values were normalized to U6 RNA as an internal control. The data are expressed as means ± SD of three assays (*, P < 0.05) and are presented as fold increases relative to naive mice (day 0), set at 1-fold. (B) The miRNA-enriched RNA was analyzed for miR-21 and miR-181b expression using a Northern blot assay kit. The results are representative of three assays. (C) miRNA-enriched RNA was isolated from Gr1⁺ CD11b⁺ cells that were purified and pooled (n = 6 mice per group) from bone marrow cells from normal and septic mice and then analyzed as for panel A.