FIG 3.

miR-21 and miR-181b antagomiRs reduce the numbers of Gr1⁺ CD11b⁺ cells in late sepsis. Bone marrow cells were harvested from moribund (the antagomiR group) and surviving (the mutant antagomiR group) mice that were sacrificed at days 10 to 21 after CLP (i.e., during late sepsis). (A) Example of flow cytometry of cells gated on Gr1⁺ CD11b⁺ staining. (B) Quantitative analysis of total Gr1⁺ CD11b⁺ cells. The data are expressed as means and SD of 5 mice per group (*, P < 0.05). (C) Percentages of CD31⁺ cells within the total Gr1⁺ CD11b⁺ cell population. The data are expressed as means and SD of 7 mice per group (*, P < 0.05). (D) Differentiation of Gr1⁺ CD11b⁺ cells. Gr1⁺ CD11b⁺ cells were differentiated for 6 days with M-CSF and rIL-4 (10 ng/ml/each). The percentages of F4/80⁺ CD11b⁺ (macrophage) and CD11c⁺ MHC-II⁺ (dendritic) cells were determined by flow cytometry. The data are expressed as means and SD of 8 mice per group (*, P < 0.05).