FIG 4.

miR-21 and miR-181b antagomiRs restore responsiveness of late-septic mice to bacterial LPS. At days 12 to 21 after CLP, mice that were moribund (the antagomiR group) and a corresponding number of surviving, healthy-appearing mice (the mutant antagomiR group) were challenged (i.p.) with 10 μg of bacterial LPS. A group of sham-operated mice also received LPS and served as a positive control. After 6 h, blood was collected and sera were obtained and used to determine the circulating levels of pro- and anti-inflammatory cytokines by ELISA. The data are expressed as means and SD of 8 mice per group. *, P < 0.05 compared with CLP plus mutant antagomiRs; **, P < 0.05 compared with sham operated.