FIG 5.

Expression of transcription factors C/EBPβ and NFI-A is upregulated during sepsis. Shown is Western blot analysis of proteins known to affect myeloid cell differentiation and/or maturation. (A) Whole bone marrow cells were pooled (n = 5 per group) from normal and septic mice that were moribund and sacrificed at days 3 and 4 (representing early sepsis) and days 10 to 14 (representing late sepsis). (B) Purified bone marrow cells Gr1⁺ CD11b⁺ cells from 5 healthy, 7 early-septic, and 5 late-septic mice were pooled. Cell extracts were prepared and probed for the indicated proteins. Only a sample of β-actin is shown.