FIG 2.

Diagrammatic representation of the CcpA protein and effect of the ccpA nonsense mutation on growth of TX82. (A) Schematic representation of the CcpA protein indicating conserved domains. (B) Amino acid sequence of CcpA of TX82 indicating the position of the nonsense mutation. (C) Growth curve of wild-type E. faecium strain TX82, the previously described acm allelic replacement mutant (TX6051), and TX6051 with a corrected ccpA gene (TX6130). Cells were grown in BHI broth at 37°C, and aliquots were removed at 0, 2, 4, 6, 9, and 24 h for determination of their CFU counts on BHI agar. Data are representative of two independent experiments.