FIG 1.

Characterization of EhMIF. (A) Pairwise amino acid sequence alignment of human MIF (HuMIF) and Entamoeba histolytica MIF (EhMIF). Sequence alignment was performed using Needle (EMBOSS). Symbols: asterisks, identical residues; colons, conserved residues; periods, semiconserved residues. (B) Secondary structure homology between HuMIF and EhMIF. The secondary structure elements of HuMIF and EhMIF are shown above and below the alignment, respectively. The figure was generated with ClustalW2 and ESPript. (C) Purified recombinant EhMIF (2 μg) in an SDS-PAGE gel stained with Coomassie Blue (lane 1), with an estimated molecular mass of 12 kDa, and immunoblot analysis of purified recombinant EhMIF (25 ng), using anti-penta-His–HRP conjugate (lane 2).