FIG 5.

Anti-EhMIF antibodies are present in children's sera. (A) ELISA with sera from children exposed to E. histolytica. Children's sera were added to ELISA plates coated with GST-EhMIF or GST only. There was a statistically significant difference between the GST-EhMIF assay (mean = 0.28; SD = 0.13) and the GST control assay (mean = 0.13; SD = 0.05) (P < 0.0001; paired-sample t test). (B) Serum positive for anti-EhMIF antibody recognized EhMIF and did not cross-react with human MIF. Human (Hu) and E. histolytica (Eh) recombinant MIF (rMIF) proteins were analyzed at 100 ng and 50 ng by immunoblotting with anti-EhMIF antibody-positive and -negative sera.