FIG 3.

CDCA and DCA repress HIF-1α protein in CF and non-CF airway cells without affecting HIF-1β protein levels. The levels of the HIF-1α, HIF-1β, and HSC70 (loading control) proteins in CF-affected IB3-1 cells (A), CFTR-corrected S9 cells (B), and A549 airway epithelial cells (C) that were untreated (control) or treated with sodium salts of CDCA or DCA at a concentration of 50 μM for 16 h are shown. Western blot data represent those from one of three independent experiments with comparable results.