FIG 5.

CDCA and DCA lead to concentration-dependent HIF-1α protein degradation through the PHD pathway and 26S proteasome machinery. The levels of HIF-1α and HSC70 (loading control) proteins in IB3-1 airway epithelial cells that were untreated (control) or treated with the PHD inhibitor DMOG (2 mM and 0.2 mM) (A), DP (100 μM and 25 μM) (B), or CoCl₂ (150 μM and 25 μM) (C) or with the 26S proteasome inhibitor MG132 (1 μM) (D) or MG101 (25 μM) (E), alone or in association with treatment with the sodium salts of CDCA or DCA at a concentration of 50 μM for 16 h, are shown. Western blot data represent those from one of three independent experiments with comparable results.