FIG 7.

(A) ELISA analysis of IB3-1 cytokine production at 3 h postinfection with LPS from P. aeruginosa serotype 10 (Sigma-Aldrich). #, values that were below the limit of detection of the ELISA kit. Statistical significance was assessed via an unpaired, two-tailed Student t test with the Welch correction. *, P < 0.05. Data presented represent those from three independent replicates. (B) Levels of HIF-1α and HSC70 (loading control) proteins in IB3-1 airway epithelial cells untreated (control) or treated with LPS (Sigma-Aldrich) at 0.1 or 10 μg/ml after 4 h or 16 h. Western blot data represent those from one of three independent experiments with comparable results.