TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| F. tularensis | ||
| SCHU S4 | F. tularensis subsp. tularensis | FSCa |
| ΔpdpC | SCHU S4 with in-frame deletion of pdpC codons 6–1325 | This study |
| ΔiglC | SCHU S4 with in-frame deletion of iglC codons 28–205 | 10 |
| ΔFTL0439 | SCHU S4 with partial deletion of ORFs fupA and fupB, identical to ORF FTL0439 in LVS | This study |
| ΔpdpC ΔpdpE | SCHU S4 with in-frame deletion of ORFS FTT1354-FTT1355 and FTT1709-FTT1710 | This study |
| ΔpdpE | SCHU S4 with in-frame deletion of ORFS FTT1355 and FTT1710 | This study |
| FSC043 | Spontaneous mutant of SCHU S4 | 10 |
| pdpC pdpE | FSC043 complemented with pdpC and pdpE genes in trans | This study |
| fupA fupB | FSC043 complemented with fupA and fupB genes in cis | This study |
| E. coli | ||
| S17-1λpir | recA thi pro hsdR hsdM+, Smr <RP4:2-Tc:Mu:Ku:Tn7> Tpr | 54 |
| Plasmids | ||
| pDMK3 | pDM4 derivative; Kmr | 55 |
| pFNLTP6groGFP | pFNL10 derivative; Kmr Cbr | 56 |
| pKK289Km | pKK214 derivative carrying gfp; Kmr | 27 |
a
FSC, Francisella Strain Collection, Swedish Defense Research Agency, Umeå, Sweden.