FIG 4.
Influence of the flagellar regulatory cascade and the Rcs system over shlBA transcriptional expression. Overnight cultures of the S. marcescens wt/ppromshlBA-gfp, fliA/ppromshlBA-gfp, flhD/ppromshlBA-gfp, wecG/ppromshlBA-gfp, and rcsB/ppromshlBA-gfp strains grown in LB medium were diluted in fresh LB medium and incubated in a 96-well black microtiter plate at 30°C without agitation for 18 h. Fluorescence (λexc = 485 nm; λem = 528 nm) and OD600 readings were determined every 60 min, with a 96-microwell Synergy2 or BioTek ELx808 microplate reader, respectively. The means and standard deviations for three independent assays performed in duplicate in each case were calculated. Statistical analysis was performed using one-way ANOVA and the Tukey-Kramer multiple-comparison test. ***, P < 0.001; statistically significantly different from wild-type S. marcescens.