FIG 10.

The CpxR D51E mutation results in increased synthesis of virulence proteins in the efp mutant. (A) Virulence proteins were detected by Western blotting using monkey convalescent-phase antiserum to S. flexneri proteins. Whole-cell lysates of equivalent numbers of cells of wild-type 2457T (WT), CFS100, which lacks the virulence plasmid (ΔpVir), the efp mutant HMS100 (Δefp), and the Δefp cpxR D51E mutant (Δefp, cpxR*) were used. The positions of the major virulence antigens and the major outer membrane protein are shown on the right. (B) The same samples as in panel A were probed with a monoclonal antibody against IpaC or a polyclonal antiserum against IcsA to confirm the reduced amounts of the proteins.