TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli strains | ||
| JW4116 | ΔpoxA::Kan | Keio collection; 27 |
| JW4107 | Δefp::Kan | Keio collection |
| JW4106 | ΔyjeK::Kan | Keio collection |
| JW5381 | ΔyfcM::Kan | Keio collection |
| S. flexneri strains | ||
| 2457T | Wild type | Walter Reed Institute of Research |
| CFS100 | 2457T ΔpVir | C. Fisher |
| HMS100 | 2457T Δefp::Kan | This work |
| HMS110 | 2457T ΔpoxA::Kan | This work |
| HMS120 | 2457T yjeK Δ202-1007::Kan | This work |
| HMS130 | 2457T ΔyfcM::Kan | This work |
| HMS101 | HMS100 encoding CpxR D51E | This work |
| HMS140 | 2457T ΔcpxA::Kan | This work |
| Plasmids | ||
| pWKS30 | Low-copy-number vector; Ampr | 41 |
| pWSc-1 | sacB gene cloned into the SmaI site of pWKS30 | 42 |
| pWSc-1 Ts | Temperature-sensitive pWSc-1 | This work |
| pPoxA | poxA cloned into EcoRV site of pWKS30 | This work |
| pEfp | efp cloned into EcoRV site of pWKS30 | This work |
| pYjeK | yjeK cloned into EcoRV site of pWKS30 | This work |