TABLE 2.

Proteins differentially expressed between wild-type 2457T and the efp or poxA mutant^a

Gene name	efp mutant/2457T expression ratiob	poxA mutant/2457T expression ratiob	Annotated function	Polyproline motif(s)	Identifier
yhbS	−5.1	−2.2	Predicted acetyltransferase		GI:24114447
yieF	−4.8	−4.5	Chromate reductase, flavoprotein		GI:332764088
fkpA	−4.5	−3.8	Heat shock peptidyl-prolyl isomerase; also has a chaperone function	PPE, PPN	GI:24114611
atpD	−3.8	−3	Beta subunit of ATP synthase	PPG	GI:112791342
fabG	−3.1	−1.9	Reductase involved in fatty acid biosynthesis		GI:333004903
ipaC	−2.9	−2.5	Secreted invasion protein		GI:281603882
rplI	−2.9	1	Ribosomal subunit protein L9		GI:15804792
acnB	−2.7	−1.2	Bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase	PPA, PPG, APP	GI:56479606
typA (bipA)	−2.5	−1.8	Ribosome binding GTPase		GI:61248993
ipaA	−2.5	−1.7	Secreted virulence effector protein		GI:31983589
mreB	−2.5	−1.3	Rod shape determination	PPE, PPG	GI:332752454
manX	−2.4	−1.2	Mannose-specific PTS component		GI:333003654
ospC2	−2.3	−1.4	Unknown function; secreted by T3SS		GI:31983556
apy/phoN2	−2.1	−2.1	Periplasmic phosphatase, apyrase		GI:31983594
infC	−2.1	−1.9	Translation initiation factor IF-3	PPV	GI:39931271
iucB	−2	−1.6	Siderophore biosynthesis	PPRP	GI:74313968
iucD	−2	−1.6	Siderophore biosynthesis	PPC, PPA	GI:332764119
mrp	−2	−1.6	ATP-binding protein involved in chromosome partitioning	PPG	GI:30063550
rpe	−1.5	6.1	Ribulose phosphate-3-epimerase		GI:332749517
afeA	−1.2	−2	Protein folding catalyst		GI:333003122
ilvE	2.7	−1.2	Branched-chain amino acid aminotransferase	PPA, PPF	GI:332751003
hslU	8.4	8.1	ATP-dependent protease ATP-binding subunit	PPA, PPG	GI:82778893

^aSpots were identified using MALDI-TOF mass spectroscopy. The identifier listed corresponds to the protein ID in GenBank. Proteins encoded on the virulence plasmid are shown in bold.

^bThe relative amount of each spot was determined, and ratios are shown for the mutants compared to the wild type by 2D-DIGE. Negative numbers indicate that smaller amounts of the protein were present in the mutant.