FIG 4.
Effects of oligopeptide transporters on the regulation of sporulation-specific gene transcription. qRT-PCR analysis of spo0A (A), the Spo0A-dependent gene murG (B), sigF (C), the σF-dependent gene gpr (D), sigE (E), the σE-dependent gene spoIID (F), sigG (G), the σG-dependent gene sspA (H), and sigK (I) expression in 630Δerm, MC296 (opp), MC301 (app), and MC307 (opp app) grown in 70:30 sporulation medium to OD600s of 0.5 (log phase) and 1.0 (start of stationary phase; T0) and 2, 4, and 8 h after T0 (T2, T4, and T8, respectively), as described in Materials and Methods. The means and standard errors of the means of at least three biological replicates are shown. WT, wild type. *, P ≤ 0.05 by a two-tailed Student's t test.