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Secretion of Cfp4 protein by three divergent phylogenetic lineages of Histoplasma yeasts. Culture filtrates collected from NAm1 strains (Hc06 and Hc20), NAm2 strains (Hc01, Hc10, Hc16, Hc22, Hc27, G217B), and the Panama strain (G186A) were treated with PNGase, and the proteins were separated by one-dimensional SDS-PAGE followed by immunoblotting with 2D20 and 2F9 antibodies.
