FIG 5.

Immunoreactivity of Cfp4 in humans with histoplasmosis. Immunoblots of culture filtrates collected from Cfp4-producing (CFP4; OSU37) and Cfp4-depleted (CFP4-RNAi; OSU87) Histoplasma yeasts using sera from patients with histoplasmosis, healthy individuals (normal human sera [NHS]from residents of Indiana), and pooled high-titer sera from patients with blastomycosis. Culture filtrate proteins (500 ng total protein) were treated with PNGase F and separated by electrophoresis for immunoblotting. The yeast-histoplasmin and mycelial-histoplasmin complement fixation titers (CF-Y and CF-M, respectively) of individual histoplasmosis patient serum are listed below each immunoblot. “nd” indicates no detection by complement fixation tests.