FIG 2.
S. mitis but not EP bacterial numbers are controlled in the chambers: association with biofilm formation. (A) Bacterial suspensions were inoculated at 1 × 109 to 2 × 109 cells/chamber at 0 h, and chamber contents were collected at different times after infection as indicated. Live bacteria were quantified as described in Materials and Methods. **, P < 0.01 compared to 0 h by 1-way ANOVA. (B to D) Titanium wires were collected from infected chambers at 72 h after infection and analyzed by scanning electron microscopy. (B) Wire only, no bacteria. (C) Wire from EP-infected chamber. (D) Wire from S. mitis-infected chamber. Bars, 100 μm (upper panels) or 2 μm (lower panels).