FIG 5.

Neutrophil function is suppressed in EP-infected chambers. Phagocytosis and ROS production were measured by FACS in Ly6G⁺ cells. (A) Phagocytosis was determined using pHrhodo-labeled E. coli, and specific phagocytosis was calculated as described in Materials and Methods. (B) Production of reactive oxygen species was detected using CellROX Deep Red. The geometric median fluorescence intensity (MFI) determined after 1 h of incubation at 37°C is shown. (C) Maximal ROS production in each sample was determined by treatment with CellROX Deep Red in the presence of the ROS stimulator TBHP. (D) Myeloperoxidase (MPO) activity was measured in total chamber fluid. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (by 1-way ANOVA).