FIG 5.
Characterizing GRA15II-expressing THP-1 cells. A retroviral vector containing the cDNA of the GRA15II allele fused to the sequence for the HA epitope tag was generated (GRA15II-HA-pMX-puro). THP-1 cells were stably transduced with this vector (GRA15II-HA) or the parental pMX-puro vector (pMXpuro) as a control. (A) Lysates were generated from stably transduced cells, and the expression of GRA15II-HA was examined by immunoblotting (IB) with an anti-HA monoclonal antibody or anti-GAPDH as a loading control. (B) Stably transduced cells were collected and stained with control Ig (gray) or anti-CD40 (white) monoclonal antibodies. In each histogram plot, the numbers in the upper right corner represent the mean fluorescence intensity of CD40 expression. One representative set of results from four independent experiments from two independent transductions is shown for both panels.