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CD40 stimulation of T. gondii-infected macrophages. BMdMs were mock infected or infected with type I, type II, or type I GRA15II parasites. At 18 hpi, 293T cells or CD40L-expressing 293T cells were added to BMdMs at a ratio of 1:2 (293T cells/BMdMs). At 26 hpi, the IL-12p70 released into the culture medium was analyzed by ELISA. The error bars represent the standard deviations of biological triplicates. n.d., not detected. **, P < 0.01 (Student's t test).
