TABLE 1.
S. pneumoniae strains used in this study
| S. pneumoniae strain | Description or characteristic(s) | Source or reference |
|---|---|---|
| D39 | Serotype 2 | NCTC7466 |
| ΔadcA mutant | 9 | |
| ΔphtABDE ΔadcAII mutant | 9 | |
| ΔphtABDE ΔadcA mutant | 9 | |
| ΔphtBDE ΔadcA mutant | Expresses only PhtA of the four Pht proteins | This study |
| ΔphtADE ΔadcA mutant | Expresses only PhtB of the four Pht proteins | This study |
| ΔphtABE ΔadcA mutant | Expresses only PhtD of the four Pht proteins | This study |
| ΔphtABD ΔadcA mutant | Expresses only PhtE of the four Pht proteins | This study |
| PhtD 3 Ala ΔadcA mutant | D39 ΔphtABDE mutant complemented with phtD with amino acid substitutions R26A, H27A, and Q28A; adcA replaced by chloramphenicol acetyltransferase gene | 19 |
| PhtE 3 Ala ΔadcA mutant | D39 ΔphtABDE mutant complemented with phtE with amino acid substitutions Q27A, H28A, and R29A; adcA replaced by chloramphenicol acetyltransferase gene | 19 |
| ΔadcAII mutant | 9 |