TABLE 2.
Oligonucleotide primers used for qRT-PCR in this study
| Primera | Sequence (5′→3′) |
|---|---|
| 16s_F | CATGCAAGTAGAACGCTGAA |
| 16s_R | TGTCATGCAACATCCACTCT |
| phtA_F | AGTCTGAAAGCCAATGCAACAG |
| phtA_R | CCTTACTTACAGATGAAGGATTAC |
| phtB_F | CCAACAGAGGAACCAGAAGAAG |
| phtB_R | TAATTGGATTCTGGATTTTTCC |
| phtD_F | GTATTAGACAAAATGCTGTGGAG |
| phtD_R | CTGTATAGGAGTCGGTTGACTTTC |
| phtE_F | GGAACAGTTGAGAACCAACCA |
| phtE_R | TAGGGTCACTCCCCACATTC |
a
The direction of the primer is indicated by the letter at the end of the primer designation as follows: F, forward; R, reverse.