FIG 1.

SDS-PAGE protein profiles of OMVs isolated under two different conditions. S. Typhimurium OMV fractions isolated under the LB (A) and acidic MgM (B) growth conditions were further purified through density gradient ultrafiltration, and the resultant gradient fractions were loaded onto SDS-PAGE gels for electrophoresis. The whole gradient was divided into nine fractions from top to bottom, and a portion of each fraction was loaded from lanes 1 to 9. The proteins were visualized through silver staining and compared.