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. 2014 Oct;82(10):4233–4240. doi: 10.1128/IAI.01880-14

FIG 4.

FIG 4

Internalization and dissemination of B. pseudomallei by DC. The internalization of B. pseudomallei (Bps) by skin DC at the footpad infection site and the dissemination of B. pseudomallei to the spleen was determined by quantifying the bacterial burden of DC (CD11c+) and non-DC (CD11c−) fractions for each organ. (A) Skin DC at the site of infection internalized B. pseudomallei. The ratio of intracellular B. pseudomallei within skin DC (Bps:103 cells) at the site of infection was significantly higher than non-DC at 2 h postinfection. (B) Dissemination of B. pseudomallei within DC to the spleen was observed at 24 and 48 h postinfection. At 48 h postinfection, the ratio of intracellular B. pseudomallei within DC (Bps:103 cells) was significantly higher than non-DC in the spleen. The increased ratio of B. pseudomallei to spleen DC (Bps:103 cells), at 48 h postinfection, indicates intracellular replication of B. pseudomallei within spleen DC. Bars depict mean ± SEM of three experiments. *, P ≤ 0.05.