FIG 3.

Examining host-parasite interactions in vivo. (A) Ai6 mice were infected i.p. with 10⁴ control Pru-mCherry or Pru-Cre-mCherry parasites, and PECs were analyzed by flow cytometry at 5 dpi. The expression of ZsGreen1 was limited to the Pru-Cre-mCherry strain. (B) PECs from Ai6 mice infected i.p. with 10⁴ Pru-Cre-tdTomato parasites were imaged at a magnification of ×40 using the ImageStream. Toxo⁺ ZsGreen1⁻ populations containing either punctate or diffuse tdTomato fluorescence were detected along with Toxo⁺ ZsGreen1⁺ and U-I populations.