FIG 4.
Kinetics of host-parasite interactions at the site of infection. (A) Ai6 mice were infected with 104 Pru-Cre-tdTomato parasites i.p., and the PECs were subsequently analyzed by flow cytometry at 3, 5, and 10 dpi. Innate cell populations were first gated through singlets, live cells, and dump− (CD3−, CD19−, NK1.1−). Further division into subsets allowed for differentiation of DCs (CD11c+, MHCII+), monocytes (non-DCs, CD11b+, Ly6G−, Ly6C+), resident macrophages (non-DCs, CD11bHI, Ly6G−, Ly6C−), and neutrophils (non-DCs, CD11b+, Ly6G+, Ly6Cint). The interactions of each of these subsets with the parasite were analyzed by plotting Toxo-tdTomato versus ZsGreen1 fluorescence. The average percentage of each quadrant is shown. (B) Quantitation of the percentage and number of cells of each fluorescent quadrant, i.e., Toxo+ ZsGreen1− (Toxo+), Toxo+ ZsGreen1+ (DP), and Toxo− ZsGreen1+ (U-I), versus days postinfection shows the kinetics of each type of host-parasite interaction at the site of infection. (n = 3 for each time point; averages ± standard errors of the means [SEM] are shown).