FIG 5.

Effect of nutrient availability on hysA regulation. (A) Growth curve of LAC-WT grown in CDM or in CDM lacking isoleucine for the first 5 h. Cells were harvested by centrifugation, washed 1 time in PBS, and suspended in either fresh CDM or CDM containing 5× BCAAs. Arrows, times when samples were taken or where medium was switched, as indicated. (B) Hyaluronidase specific activity of samples taken at the time points indicated in panel A. Statistical analysis was performed using Student's t test (****, P < 0.0001). (C) Immunoblot for the LukS subunit of PVL at the time points indicated in panel A. (D) Quantification of LukS immunoblot using ImageJ software. post, samples taken at time points after the medium switch.