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HysA is required for the breakdown of hyaluronic acid in vivo. (A and B) Micrographs of immunofluorescently labeled HA in the lungs of mice inoculated with 1.0 × 107 CFU of either strain LAC-WT or its ΔhysA mutant strain. (C) The mean fluorescence intensity was quantified for each sample (40 fields per section). A sample treated with hyaluronidase from Streptomyces hyalurolyticus (Strep Hys) was also included as a control. NS, not significant; ****, P < 0.0001.
