Strains |
|
|
E. coli
|
|
|
BW25141 |
Cloning strain |
58 |
ER2566 |
Protein expression strain |
New England Biolabs |
DH5α |
Cloning/protein expression strain |
Gibco |
AH2856 |
HysA protein expression strain, ER2566 containing pCR03 |
This work |
S. aureus
|
|
|
RN4220 |
Restriction-deficient strain 8325-4 |
59 |
AH1263 |
USA300 CA-MRSA Erms (LAC) |
39 |
AH2525 |
AH1263 ΔhysA
|
This work |
AH2839 |
AH2525 containing pHysA |
This work |
JE2 |
CA-MRSA USA300 Erms, plasmid-cured LAC derivative |
40 |
AH2914 |
JE2 hysA::ΦNΣ Ermr
|
40 |
AH2936 |
JE2 sarA::ΦNΣ Ermr
|
40 |
AH2937 |
JE2 sigB::ΦNΣ Ermr
|
40 |
AH2938 |
JE2 rsbW::ΦNΣ Ermr
|
40 |
AH2939 |
JE2 rsbU::ΦNΣ Ermr
|
40 |
AH2942 |
JE2 SAUSA300_0543::ΦNΣ Ermr
|
40 |
AH2945 |
JE2 hslU::ΦNΣ Ermr
|
40 |
AH2946 |
JE2 codY::ΦNΣ Ermr
|
40 |
AH3052 |
AH1263 Δspa
|
This work |
AH3134 |
AH1263 Δspa codY::ΦNΣ Ermr
|
This work |
AH3207 |
AH1263 Δspa codY::ΦNΣ Tetr
hysA::ΦNΣ Ermr
|
This work |
AH1292 |
AH1263 Δagr::tetM
|
60 |
AH2032 |
AH1263 Δagr::tetM ΔsigB
|
60, 61
|
AH1483 |
AH1263 ΔsigB
|
62 |
AH3453 |
AH1263 Δagr::tetM codY::ΦNΣ Ermr
|
This work |
AH3172 |
AH1263 ΔhysA codY::ΦNΣ Ermr
|
This work |
Plasmids |
|
|
pET28a |
E. coli protein expression vector |
Novagen |
pJB38 |
S. aureus gene knockout vector, Camr
|
31 |
pTet |
Antibiotic cassette replacement vector, Camr Ampr
|
32 |
pSKerm-MCS |
E. coli-S. aureus shuttle vector, Ermr Camr
|
63 |
pALC2073 |
E. coli-S. aureus shuttle vector, Camr
|
64 |
pALC2073-RNAIII |
Tetracycline-inducible RNAIII expression vector, Camr
|
60 |
pCR02 |
pJB38 hysA knockout vector, Camr
|
This work |
pHysA (pCR01) |
hysA complementation plasmid, Camr Ermr
|
This work |
pCR03 |
hysA 6× His-tagged E. coli protein expression vector, Kanr
|
This work |
pCR04 |
pCR01 mutated CodY box, Camr Ermr
|
This work |
pCR05 |
pCR01 mutated CodY box, Camr Ermr
|
This work |
pCR06 |
pCR01 mutated CodY box, Camr Ermr
|
This work |