TABLE 1.

Strains and plasmids

Strain or plasmid	Description	Reference(s) or source
Strains
E. coli
BW25141	Cloning strain	58
ER2566	Protein expression strain	New England Biolabs
DH5α	Cloning/protein expression strain	Gibco
AH2856	HysA protein expression strain, ER2566 containing pCR03	This work
S. aureus
RN4220	Restriction-deficient strain 8325-4	59
AH1263	USA300 CA-MRSA Erms (LAC)	39
AH2525	AH1263 ΔhysA	This work
AH2839	AH2525 containing pHysA	This work
JE2	CA-MRSA USA300 Erms, plasmid-cured LAC derivative	40
AH2914	JE2 hysA::ΦNΣ Ermr	40
AH2936	JE2 sarA::ΦNΣ Ermr	40
AH2937	JE2 sigB::ΦNΣ Ermr	40
AH2938	JE2 rsbW::ΦNΣ Ermr	40
AH2939	JE2 rsbU::ΦNΣ Ermr	40
AH2942	JE2 SAUSA300_0543::ΦNΣ Ermr	40
AH2945	JE2 hslU::ΦNΣ Ermr	40
AH2946	JE2 codY::ΦNΣ Ermr	40
AH3052	AH1263 Δspa	This work
AH3134	AH1263 Δspa codY::ΦNΣ Ermr	This work
AH3207	AH1263 Δspa codY::ΦNΣ Tetr hysA::ΦNΣ Ermr	This work
AH1292	AH1263 Δagr::tetM	60
AH2032	AH1263 Δagr::tetM ΔsigB	60, 61
AH1483	AH1263 ΔsigB	62
AH3453	AH1263 Δagr::tetM codY::ΦNΣ Ermr	This work
AH3172	AH1263 ΔhysA codY::ΦNΣ Ermr	This work
Plasmids
pET28a	E. coli protein expression vector	Novagen
pJB38	S. aureus gene knockout vector, Camr	31
pTet	Antibiotic cassette replacement vector, Camr Ampr	32
pSKerm-MCS	E. coli-S. aureus shuttle vector, Ermr Camr	63
pALC2073	E. coli-S. aureus shuttle vector, Camr	64
pALC2073-RNAIII	Tetracycline-inducible RNAIII expression vector, Camr	60
pCR02	pJB38 hysA knockout vector, Camr	This work
pHysA (pCR01)	hysA complementation plasmid, Camr Ermr	This work
pCR03	hysA 6× His-tagged E. coli protein expression vector, Kanr	This work
pCR04	pCR01 mutated CodY box, Camr Ermr	This work
pCR05	pCR01 mutated CodY box, Camr Ermr	This work
pCR06	pCR01 mutated CodY box, Camr Ermr	This work