FIG 1.

Neutrophil recruitment following PA103 infection requires IL-1R- and MyD88-dependent signaling but is independent of caspase-1 or caspase-11. Mice of the indicated genotypes were intranasally infected with ca. 5 × 10⁴ CFU of PA103 and then euthanized at 4 hpi. (A) Ratio of the number of CFU recovered from lung/number of CFU in the inoculum; (B) total cell count in BAL fluid; (C) number of neutrophils (polymorphonuclear leukocytes [PMN]) in BAL fluid. Each symbol represents one mouse; bars indicate the geometric mean (A) or median (B, C) for each group. Statistical significance was tested by analysis of variance (Kruskal-Wallis test), followed by Dunn's multiple-comparison test. *, P < 0.5; **, P < 0.01; ***, P < 0.001.